DEF6 Mouse Monoclonal Antibody [Clone ID: OTI1E3]

SKU CF505321

Carrier-free (BSA/glycerol-free) DEF6 mouse monoclonal antibody, clone OTI1E3 (formerly 1E3)

Product Data

• Clone Name: OTI1E3
• Application: IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100
• Reactivity: Dog, Human, Monkey, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DEF6(NP_071330) produced in HEK293T cell.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 73.7 kDa
• Gene Name: DEF6 guanine nucleotide exchange factor
• Database Link:
  NP_071330
  Q9H4E7
• Background: DEF6, or IBP, is a guanine nucleotide exchange factor (GEF) for RAC (MIM 602048) and CDC42 (MIM 116952) that is highly expressed in B and T cells (Gupta et al., 2003 [PubMed 12923183]). [supplied by OMIM]
• Synonyms: IBP; SLAT; SWAP70L

Reference Data

• Protein Families: Druggable Genome