LIM1 (LHX1) Mouse Monoclonal Antibody [Clone ID: OTI2D5]

SKU CF504527

Carrier-free (BSA/glycerol-free) LHX1 mouse monoclonal antibody, clone OTI2D5 (formerly 2D5)

Product Data

• Clone Name: OTI2D5
• Application: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~1000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivity: Human, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 100-362 of human LHX1(NP_005559) produced in HEK293T cell.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 44.6 kDa
• Gene Name: LIM homeobox 1
• Database Link:
  NP_005559
  P48742
• Background: This gene encodes a member of a large protein family which contains the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein is a transcription factor important for the development of the renal and urogenital systems. This gene is a candidate for Mayer-Rokitansky-Kuster-Hauser syndrome, a disorder characterized by anomalies in the female genital tract. [provided by RefSeq]
• Synonyms: LIM-1; LIM1

Reference Data

• Protein Families: ES Cell Differentiation/IPS, Transcription Factors