GADD34 (PPP1R15A) Mouse Monoclonal Antibody [Clone ID: OTI3D12]

SKU CF504310

Carrier-free (BSA/glycerol-free) PPP1R15A mouse monoclonal antibody, clone OTI3D12 (formerly 3D12)

Product Data

• Clone Name: OTI3D12
• Application: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivity: Dog, Human, Monkey, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PPP1R15A(NP_055145) produced in HEK293T cell.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 73.3 kDa
• Gene Name: protein phosphatase 1 regulatory subunit 15A
• Database Link:
  NP_055145
  O75807
• Background: This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation. [provided by RefSeq, Jul 2008]
• Synonyms: GADD34

Reference Data

• Protein Families: Druggable Genome