NAPE PLD (NAPEPLD) Mouse Monoclonal Antibody [Clone ID: OTI5F7]

SKU CF503860

Carrier-free (BSA/glycerol-free) NAPEPLD mouse monoclonal antibody, clone OTI5F7 (formerly 5F7)

Product Data

• Clone Name: OTI5F7
• Application: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivity: Human, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NAPEPLD(NP_945341) produced in HEK293T cell.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 45.4 kDa
• Gene Name: N-acyl phosphatidylethanolamine phospholipase D
• Database Link:
  NP_945341
  Q6IQ20
• Background: NAPEPLD is a phospholipase D type enzyme that catalyzes the release of N-acylethanolamine (NAE) from N-acyl-phosphatidylethanolamine (NAPE) in the second step of the biosynthesis of N-acylethanolamine (Okamoto et al., 2004 [PubMed 14634025]). [supplied by OMIM, Oct 2008]
• Synonyms: FMP30; NAPE-PLD

Reference Data