HARS2 Mouse Monoclonal Antibody [Clone ID: OTI5H2]

SKU CF503834

Carrier-free (BSA/glycerol-free) HARS2 mouse monoclonal antibody, clone OTI5H2 (formerly 5H2)

Product Data

• Clone Name: OTI5H2
• Application: FC, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, FLOW 1:100
• Reactivity: Dog, Human, Monkey
• Antibody Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human HARS2(NP_036340) produced in HEK293T cell.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 53.3 kDa
• Gene Name: histidyl-tRNA synthetase 2, mitochondrial
• Database Link:
  NP_036340
  P49590
• Background: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is an enzyme belonging to the class II family of aminoacyl-tRNA synthetases. Functioning in the synthesis of histidyl-transfer RNA, the enzyme plays an accessory role in the regulation of protein biosynthesis. The gene is located in a head-to-head orientation with HARS on chromosome five, where the homologous genes share a bidirectional promoter. [provided by RefSeq]. COMPLETENESS: full length.
• Synonyms: HARSL; HARSR; HO3; PRLTS2

Reference Data

• Protein Pathways: Aminoacyl-tRNA biosynthesis