PNPO Mouse Monoclonal Antibody [Clone ID: OTI1G9]

SKU CF503506

Carrier-free (BSA/glycerol-free) PNPO mouse monoclonal antibody, clone OTI1G9 (formerly 1G9)

Product Data

• Clone Name: OTI1G9
• Application: FC, IHC, WB
• Recommended Dilution: WB 1:4000, IHC 1:150, FLOW 1:100
• Reactivity: Human, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PNPO(NP_060599) produced in HEK293T cell.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 29.8 kDa
• Gene Name: pyridoxamine 5'-phosphate oxidase
• Database Link:
  NP_060599
  Q9NVS9
• Background: The enzyme encoded by this gene catalyzes the terminal, rate-limiting step in the synthesis of pyridoxal 5'-phosphate, also known as vitamin B6. Vitamin B6 is a required co-factor for enzymes involved in both homocysteine metabolism and synthesis of neurotransmitters such as catecholamine. Mutations in this gene result in pyridoxamine 5'-phosphate oxidase (PNPO) deficiency, a form of neonatal epileptic encephalopathy. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: HEL-S-302; PDXPO

Reference Data

• Protein Pathways: Metabolic pathways, Vitamin B6 metabolism