Apg3 (ATG3) Mouse Monoclonal Antibody [Clone ID: OTI2C12]

CAT#: CF503161

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Carrier-free (BSA/glycerol-free) ATG3 mouse monoclonal antibody, clone OTI2C12 (formerly 2C12)



  View other "OTI2C12" antibodies (4)

USD 600.00


Availability*
In Stock

Size
    • 100 ug


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Specifications

Product Data
Clone Name OTI2C12
Applications FC, IF, IHC, WB
Recommended Dilution WB 1:2000, IHC 1:150, IF 1:50~100, FLOW 1:100
Reactivities Human, Mouse, Rat
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Full length human recombinant protein of human ATG3(NP_057393) produced in HEK293 cell.
Formulation Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Predicted Protein Size 35.7 kDa
Gene Name Homo sapiens autophagy related 3 (ATG3), transcript variant 1, mRNA.
Background Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3, a protein-conjugating enzyme essential for autophagy (Tanida et al., 2002 [PubMed 11825910]). [supplied by OMIM]
Synonyms APG3; APG3-LIKE; APG3L; PC3-96
Reference Data
Protein Pathways Regulation of autophagy

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