AP2M1 Mouse Monoclonal Antibody [Clone ID: OTI1F10]

SKU CF503015

Carrier-free (BSA/glycerol-free) AP2M1 mouse monoclonal antibody, clone OTI1F10 (formerly 1F10)

Product Data

• Clone Name: OTI1F10
• Application: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivity: Human, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 97-383 of human AP2M1 (NP_001020376) produced in E.coli.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 49.2 kDa
• Gene Name: adaptor related protein complex 2 subunit mu 1
• Database Link:
  NP_001020376
  Q96CW1
• Background: This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
• Synonyms: AP50; CLAPM1; mu2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, Huntington's disease