eRF1 (ETF1) Mouse Monoclonal Antibody [Clone ID: OTI2E3]

SKU CF502891

Carrier-free (BSA/glycerol-free) ETF1 mouse monoclonal antibody, clone OTI2E3 (formerly 2E3)

Product Data

• Clone Name: OTI2E3
• Application: FC, WB
• Recommended Dilution: WB 1:500, FLOW 1:100
• Reactivity: Human, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ETF1 (NP_004721) produced in HEK293T cell.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 48.9 kDa
• Gene Name: eukaryotic translation termination factor 1
• Database Link:
  NP_004721
  P62495
• Background: This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in coding and non-coding transcript variants. Pseudogenes of this gene are found on chromosomes 5, 7 and X. [provided by RefSeq, Jan 2012]
• Synonyms: D5S1995; ERF; ERF1; RF1; SUP45L1; TB3-1

Reference Data