LNK (SH2B3) Mouse Monoclonal Antibody [Clone ID: OTI2D8]

SKU CF502764

Carrier-free (BSA/glycerol-free) SH2B3 mouse monoclonal antibody, clone OTI2D8 (formerly 2D8)

Product Data

• Clone Name: OTI2D8
• Application: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivity: Human
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SH2B3 (NP_005466) produced in HEK293T cell.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 63 kDa
• Gene Name: SH2B adaptor protein 3
• Database Link:
  NP_005466
  Q9UQQ2
• Background: This gene encodes a member of the SH2B adaptor family of proteins, which are involved in a range of signaling activities by growth factor and cytokine receptors. The encoded protein is a key negative regulator of cytokine signaling and plays a critical role in hematopoiesis. Mutations in this gene have been associated with susceptibility to celiac disease type 13 and susceptibility to insulin-dependent diabetes mellitus. [provided by RefSeq, May 2010]
• Synonyms: IDDM20; LNK

Reference Data

• Protein Pathways: Neurotrophin signaling pathway