NNMT Mouse Monoclonal Antibody [Clone ID: OTI3D8]

SKU CF502624

Carrier-free (BSA/glycerol-free) NNMT mouse monoclonal antibody, clone OTI3D8 (formerly 3D8)

Product Data

• Clone Name: OTI3D8
• Application: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivity: Human, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NNMT(NP_006160) produced in HEK293T cell.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 29.4 kDa
• Gene Name: nicotinamide N-methyltransferase
• Database Link:
  NP_006160
  P40261
• Background: N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor. [provided by RefSeq]
• Synonyms: nicotinamide N-methyltransferase

Reference Data

• Protein Pathways: Metabolic pathways, Nicotinate and nicotinamide metabolism