GBA3 Mouse Monoclonal Antibody [Clone ID: OTI1F1]

SKU CF502602

Carrier-free (BSA/glycerol-free) GBA3 mouse monoclonal antibody, clone OTI1F1 (formerly 1F1)

Product Data

• Clone Name: OTI1F1
• Application: FC, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, FLOW 1:100
• Reactivity: Human
• Antibody Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-150 and 370-469 of human GBA3 (NP_066024) produced in E.coli.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 53.5 kDa
• Gene Name: glucosylceramidase beta 3 (gene/pseudogene)
• Database Link:
  NP_066024
  Q9H227
• Background: GBA3, or cytosolic beta-glucosidase (EC 3.2.1.21), is a predominantly liver enzyme that efficiently hydrolyzes beta-D-glucoside and beta-D-galactoside, but not any known physiologic beta-glycoside, suggesting that it may be involved in detoxification of plant glycosides (de Graaf et al., 2001 [PubMed 11389701]). GBA3 also has significant neutral glycosylceramidase activity (EC 3.2.1.62), suggesting that it may be involved in a nonlysosomal catabolic pathway of glucosylceramide metabolism (Hayashi et al., 2007 [PubMed 17595169]). [supplied by OMIM]
• Synonyms: CBG; CBGL1; GLUC; KLRP

Reference Data

• Protein Pathways: Cyanoamino acid metabolism, Starch and sucrose metabolism