POLR2E Mouse Monoclonal Antibody [Clone ID: OTI3B5]

SKU
CF502548
Carrier-free (BSA/glycerol-free) POLR2E mouse monoclonal antibody, clone OTI3B5 (formerly 3B5)
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    Antibodies made against full-length proteins as antigens.

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$600.00
3 Days*
Specifications
Product Data
Clone Name OTI3B5
Application FC, IF, IHC, WB
Recommended Dilution WB 1:500~2000, IHC 1:150, FLOW 1:100, IF: 1:100
Reactivity Dog, Human, Monkey, Mouse, Rat
Antibody Host Mouse
Isotype IgG2a
Clonality Monoclonal
Immunogen Full length human recombinant protein of human POLR2E(NP_002686) produced in HEK293T cell.
Buffer Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Shipping Ambient
Predicted Protein Size 24.4 kDa
Gene Name RNA polymerase II, I and III subunit E
Database Link
Background This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. [provided by RefSeq]
Synonyms hRPB25; hsRPB5; RPABC1; RPB5; XAP4
Reference Data
Protein Families Transcription Factors
Protein Pathways Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
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SKU Description Size Price
TA502548 POLR2E mouse monoclonal antibody, clone OTI3B5 (formerly 3B5) 100 ul
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TA502548AM POLR2E mouse monoclonal antibody, clone OTI3B5 (formerly 3B5), Biotinylated 100 ul
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TA502548BM POLR2E mouse monoclonal antibody, clone OTI3B5 (formerly 3B5), HRP conjugated 100 ul
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TA502548S POLR2E mouse monoclonal antibody, clone OTI3B5 (formerly 3B5) 30 ul
$200.00

Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.