HSPC150 (UBE2T) Mouse Monoclonal Antibody [Clone ID: OTI2F5]

CAT#: CF501645

Carrier-free (BSA/glycerol-free) UBE2T mouse monoclonal antibody, clone OTI2F5 (formerly 2F5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY UBE2T (Cat# RC200748, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2T(Cat# TA501645). Positive lysates LY402285 (100ug) and LC402285 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-UBE2T monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Anti-UBE2T mouse monoclonal antibody (TA501645) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY UBE2T (RC200748).

Specifications

Product Data

• Clone Name: OTI2F5
• Applications: IF, WB
• Recommended Dilution: WB 1:500, IF 1:100
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human UBE2T (NP_054895) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 22.3 kDa
• Gene Name: ubiquitin conjugating enzyme E2 T
• Database Link:
  NP_054895
  Entrez Gene 67196 MouseEntrez Gene 29089 Human
  UniProt ID Q9NPD8
• Background: The covalent conjugation of ubiquitin to proteins regulates diverse cellular pathways and proteins. Ubiquitin is transferred to a target protein through a concerted action of a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), such as UBE2T, and a ubiquitin ligase (E3) (Machida et al., 2006 [PubMed 16916645]). [supplied by OMIM]
• Synonyms: FANCT; HSPC150; PIG50

Reference Data

• Protein Families: Druggable Genome