HSPC150 (UBE2T) Mouse Monoclonal Antibody [Clone ID: OTI2F5]

SKU CF501645

Carrier-free (BSA/glycerol-free) UBE2T mouse monoclonal antibody, clone OTI2F5 (formerly 2F5)

Product Data

• Clone Name: OTI2F5
• Application: IF, WB
• Recommended Dilution: WB 1:500, IF 1:100
• Reactivity: Human, Mouse
• Antibody Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human UBE2T (NP_054895) produced in HEK293T cell.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 22.3 kDa
• Gene Name: ubiquitin conjugating enzyme E2 T
• Database Link:
  NP_054895
  Q9NPD8
• Background: The covalent conjugation of ubiquitin to proteins regulates diverse cellular pathways and proteins. Ubiquitin is transferred to a target protein through a concerted action of a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), such as UBE2T, and a ubiquitin ligase (E3) (Machida et al., 2006 [PubMed 16916645]). [supplied by OMIM]
• Synonyms: FANCT; HSPC150; PIG50

Reference Data

• Protein Families: Druggable Genome