Glucose 6 phosphate isomerase (GPI) Mouse Monoclonal Antibody [Clone ID: OTI2D2]

CAT#: CF501171

Carrier-free (BSA/glycerol-free) GPI mouse monoclonal antibody, clone OTI2D2 (formerly 2D2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GPI (Cat# RC201232, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GPI(Cat# TA501171). Positive lysates LY400066 (100ug) and LC400066 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GPI monoclonal antibody.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and GPI-Knockout 293T cells (KO, Cat# LC840273 ) were separated by SDS-PAGE and immunoblotted with anti-GPI monoclonal antibody TA501171 (1:500`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-GPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501171, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-GPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501171, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-GPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501171, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-GPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501171, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-GPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501171, Dilution 1:50)

• Anti-GPI mouse monoclonal antibody (TA501171) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GPI (RC201232).

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Specifications

Product Data

• Clone Name: OTI2D2
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:1000~2000, IHC 1:50, IF 1:100
• Reactivities: Human, Monkey, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GPI(NP_000166) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 63.0 kDa
• Gene Name: Homo sapiens glucose-6-phosphate isomerase (GPI), transcript variant 2, mRNA.
• Database Link:
  NP_000166
  Entrez Gene 292804 RatEntrez Gene 717933 MonkeyEntrez Gene 2821 Human
  UniProt ID P06744
• Background: This gene belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.
• Synonyms: AMF; GNPI; NLK; PGI; PHI; SA-36; SA36

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Pentose phosphate pathway, Starch and sucrose metabolism