NIT1 Mouse Monoclonal Antibody [Clone ID: OTI4D12]

SKU CF501034

Carrier-free (BSA/glycerol-free) NIT1 mouse monoclonal antibody, clone OTI4D12 (formerly 4D12)

Product Data

• Clone Name: OTI4D12
• Application: FC, IF, IHC, WB
• Recommended Dilution: WB 1:1000, IHC 1:50, IF 1:100, Flow 1:100
• Reactivity: Human, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NIT1 (NP_005591) produced in HEK293T cell.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 35.7 kDa
• Gene Name: nitrilase 1
• Database Link:
  NP_005591
  Q86X76
• Background: Play a role in cell growth and apoptosis: loss of expression promotes cell growth and resistance to DNA damage stress. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. it is also a negative regulator of primary T-cells. Has apparently no omega-amidase activity such as NIT2.
• Synonyms: MGC57670

Reference Data