BHMT Mouse Monoclonal Antibody [Clone ID: OTI3E11]

SKU CF500959

Carrier-free (BSA/glycerol-free) BHMT mouse monoclonal antibody, clone OTI3E11 (formerly 3E11)

Product Data

• Clone Name: OTI3E11
• Application: FC, IF, IHC, WB
• Recommended Dilution: IHC 1:200, WB: 1:200
• Reactivity: Human, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human BHMT (NP_001704) produced in HEK293T cell.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 45 kDa
• Gene Name: betaine--homocysteine S-methyltransferase
• Database Link:
  NP_001704
  Q93088
• Background: This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed.
• Synonyms: BHMT1; HEL-S-61p

Reference Data

• Protein Pathways: Cysteine and methionine metabolism, Glycine, Metabolic pathways, serine and threonine metabolism