MAN1 (LEMD3) Mouse Monoclonal Antibody [Clone ID: OTI4E1]

SKU CF500797

Carrier-free (BSA/glycerol-free) LEMD3 mouse monoclonal antibody, clone OTI4E1 (formerly 4E1)

Product Data

• Clone Name: OTI4E1
• Application: IP, WB
• Recommended Dilution: WB 1:1000, IF 1:100, Flow 1:100, IP 2-4ug/mg
• Reactivity: Human, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human LEMD3 (NP_055134) produced in HEK293T cell.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 100.0 kDa
• Gene Name: LEM domain containing 3
• Database Link:
  NP_055134
  Q9Y2U8
• Background: This locus encodes a LEM domain-containing protein. The encoded protein functions to antagonize transforming growth factor-beta signaling at the inner nuclear membrane. Two transcript variants encoding different isoforms have been found for this gene. Mutations in this gene have been associated with osteopoikilosis, Buschke-Ollendorff syndrome and melorheostosis
• Synonyms: MAN1

Reference Data

• Protein Families: Transmembrane