Cytochrome P450 2E1 (CYP2E1) Mouse Monoclonal Antibody [Clone ID: OTI5B9]

CAT#: CF500734

Carrier-free (BSA/glycerol-free) CYP2E1 mouse monoclonal antibody, clone OTI5B9 (formerly 5B9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CYP2E1 (Cat# RC213502, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP2E1(Cat# TA500734). Positive lysates LY400264 (100ug) and LC400264 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-CYP2E1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500734, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-CYP2E1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500734, Dilution 1:50)

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  Anti-CYP2E1 mouse monoclonal antibody (TA500734) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CYP2E1 (RC213502).

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  Immunofluorescent staining of HepG2 cells using anti-CYP2E1 mouse monoclonal antibody (TA500734).

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Specifications

Product Data

• Clone Name: OTI5B9
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:50, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CYP2E1 (NP_000764) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 56.8 kDa
• Gene Name: cytochrome P450 family 2 subfamily E member 1
• Database Link:
  NP_000764
  Entrez Gene 1571 Human
  UniProt ID P05181
• Background: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer.
• Synonyms: CPE1; CYP2E; P450-J; P450C2E

Reference Data

• Protein Families: Druggable Genome, P450, Transmembrane
• Protein Pathways: Arachidonic acid metabolism, Drug metabolism - cytochrome P450, Linoleic acid metabolism, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450