ID3 Mouse Monoclonal Antibody [Clone ID: OTI15F4]

SKU CF500716

Carrier-free (BSA/glycerol-free) ID3 mouse monoclonal antibody, clone OTI15F4 (formerly 15F4)

Product Data

• Clone Name: OTI15F4
• Application: FC, IF, IHC, WB
• Recommended Dilution: IHC 1:100~400, WB: 1:200
• Reactivity: Human, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ID3 (NP_002158) produced in HEK293T cell.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 12.8 kDa
• Gene Name: inhibitor of DNA binding 3, HLH protein
• Database Link:
  NP_002158
  Q02535
• Background: Members of the ID family of helix-loop-helix (HLH) proteins lack a basic DNA-binding domain and inhibit transcription through formation of nonfunctional dimers that are incapable of binding to DNA. [supplied by OMIM]
• Synonyms: bHLHb25; HEIR-1

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
• Protein Pathways: TGF-beta signaling pathway