Carbonic Anhydrase IX (CA9) Mouse Monoclonal Antibody [Clone ID: OTI1G7]

SKU CF500623

Carrier-free (BSA/glycerol-free) CA9 mouse monoclonal antibody, clone OTI1G7 (formerly 1G7)

Product Data

• Clone Name: OTI1G7
• Application: FC, IHC
• Recommended Dilution: IHC 1:50, Flow 1:100
• Reactivity: Human
• Antibody Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human CA9 expression vector
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 49.7 kDa
• Gene Name: carbonic anhydrase 9
• Database Link:
  NP_001207
  Q16790
• Background: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-basebalance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12.
• Synonyms: CAIX; MN

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Nitrogen metabolism