Chk2 (CHEK2) Mouse Monoclonal Antibody [Clone ID: OTI5C4]

CAT#: CF500398

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Carrier-free (BSA/glycerol-free) CHEK2 mouse monoclonal antibody, clone OTI5C4 (formerly 5C4)



  View other "OTI5C4" antibodies (4)

USD 545.00


Availability*
In Stock

Size
    • 100 ug


Specifications

Product Data
Clone Name OTI5C4
Applications FC, IF, IHC, IP, WB
Recommended Dilution WB 1:500~1000, IHC 1:150, IF 1:50~100, FLOW 1:100, IP 2ug/500ul
Reactivities Human, Mouse, Rat, Dog
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Full length human recombinant protein of human CHEK2 (NP_009125) produced in HEK293T cell.
Formulation Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Predicted Protein Size 60.7 kDa
Gene Name Homo sapiens checkpoint kinase 2 (CHEK2), transcript variant 1, mRNA.
Background In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Synonyms CDS1; CHK2; hCds1; HuCds1; LFS2; PP1425; RAD53
Reference Data
Protein Families Druggable Genome, Protein Kinase, Stem cell - Pluripotency
Protein Pathways Cell cycle, p53 signaling pathway

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