Neurogenin3 (NEUROG3) Mouse Monoclonal Antibody [Clone ID: OTI3B5]

CAT#: CF500346

Carrier-free (BSA/glycerol-free) NEUROG3 mouse monoclonal antibody, clone OTI3B5 (formerly 3B5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NEUROG3 (Cat# RC224767, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEUROG3(Cat# TA500346). Positive lysates LY402827 (100ug) and LC402827 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-NEUROG3 mouse monoclonal antibody (TA500346) at 1:150 dilution.

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-NEUROG3 mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

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  Anti-NEUROG3 mouse monoclonal antibody (NEUROG3) immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY NEUROG3 (RC224767).

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  Immunoprecipitation of NEUROG3 by using TrueMab monoclonal anti-NEUROG3 antibody (Negative control: IP without adding anti-NEUROG3 antibody). For each experiment, 500ul of DDK tagged NEUROG3 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-NEUROG3 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

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Specifications

Product Data

• Clone Name: OTI3B5
• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB 1:2000, IHC 1:50 - 1:150, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human NEUROG3 expression vector
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 23.077 kDa
• Gene Name: neurogenin 3
• Database Link:
  NP_066279
  Entrez Gene 50674 Human
  UniProt ID Q9Y4Z2
• Background: Neurogenin-3 (NEUROG3) is expressed in endocrine progenitor cells and is required for endocrine cell development in the pancreas and intestine (Wang et al., 2006 [PubMed 16855267]). It belongs to a family of basic helix-loop-helix transcription factors involved in the determination of neural precursor cells in the neuroectoderm
• Synonyms: Atoh5; bHLHa7; Math4B; NGN-3; ngn3

Reference Data

• Protein Families: ES Cell Differentiation/IPS
• Protein Pathways: Maturity onset diabetes of the young