Neurogenin3 (NEUROG3) Mouse Monoclonal Antibody [Clone ID: OTI3B5]

SKU CF500346

Carrier-free (BSA/glycerol-free) NEUROG3 mouse monoclonal antibody, clone OTI3B5 (formerly 3B5)

Product Data

• Clone Name: OTI3B5
• Application: IF, IHC, IP, WB
• Recommended Dilution: WB 1:2000, IHC 1:50 - 1:150, IF 1:100
• Reactivity: Human
• Antibody Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human NEUROG3 expression vector
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 23.077 kDa
• Gene Name: neurogenin 3
• Database Link:
  NP_066279
  Q9Y4Z2
• Background: Neurogenin-3 (NEUROG3) is expressed in endocrine progenitor cells and is required for endocrine cell development in the pancreas and intestine (Wang et al., 2006 [PubMed 16855267]). It belongs to a family of basic helix-loop-helix transcription factors involved in the determination of neural precursor cells in the neuroectoderm
• Synonyms: Atoh5; bHLHa7; Math4B; NGN-3; ngn3
• Note: WB application: Only overexpression results are guaranteed, not endogenous.

Reference Data

• Protein Families: ES Cell Differentiation/IPS
• Protein Pathways: Maturity onset diabetes of the young