HNF6 (ONECUT1) Mouse Monoclonal Antibody [Clone ID: OTI4F12]

SKU CF500196

Carrier-free (BSA/glycerol-free) HNF6 mouse monoclonal antibody, clone OTI4F12 (formerly 4F12)

Product Data

• Clone Name: OTI4F12
• Application: IF, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:50, IF 1:100
• Reactivity: Human, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 85-198 of human HNF6 (NP_004489) produced in E.coli.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 50.8 kDa
• Gene Name: one cut homeobox 1
• Database Link:
  NP_004489
  Q9UBC0
• Background: The protein encoded by HNF6 is Transcriptional activator. Binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a varietyof gene promoters such as those of HNF3B and TTR. Important for liver genes transcription.
• Synonyms: HNF-6; HNF6; HNF6A

Reference Data

• Protein Families: ES Cell Differentiation/IPS, Transcription Factors
• Protein Pathways: Maturity onset diabetes of the young