Cytokeratin 8 (KRT8) Mouse Monoclonal Antibody [Clone ID: OTI2E4]

SKU CF500022

Carrier-free (BSA/glycerol-free) CK8 mouse monoclonal antibody, clone OTI2E4 (formerly 2E4)

Product Data

• Clone Name: OTI2E4
• Application: IF, IHC, WB
• Recommended Dilution: WB 1:500~1000, IHC 1:50, IF 1:50~100
• Reactivity: Human, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 91-381 of human CK8 (NP_002264) produced in E.coli.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 53.5 kDa
• Gene Name: keratin 8
• Database Link:
  NP_002264
  P05787
• Background: Keratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.
• Synonyms: CARD2; CK-8; CK8; CYK8; K2C8; K8; KO

Reference Data

• Protein Families: Druggable Genome