GNRHR Mouse Monoclonal Antibody [Clone ID: A9E4]

SKU BM582S

GNRHR mouse monoclonal antibody, clone A9E4, Purified

Product Data

• Clone Name: A9E4
• Application: ELISA, IHC, WB
• Recommended Dilution: ELISA. ; Western Blot. ; Flow Cytometry. ; Immunohistochemistry on Frozen Sections.
• Reactivity: Human
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide corresponding to the amino acids 1-29 of the Human GnRH receptor extracellular domain conjugated to BSA.; Splenocytes were fused with SP2/0 mouse myeloma cells. Stability was established by subcloning four times.
• Specificity: This Monoclonal antibody A9E4 reacts with GnRH receptors in the anterior pituitary.; A9E4 showed specificity for the synthetic peptide in ELISA and Dot blot, it has a higher affinity for the antigen than the GnRH receptor antibody clone F1G4 (Cat.-No. AM32064PU-N).; A9E4 specifically stains human GnRH receptors on frozen tissue of the anterior pituitary.; Recognition was supported by Western blotting.
• Buffer: PBS; State: Purified; State: Liquid purified IgG fraction; Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. ; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: gonadotropin releasing hormone receptor
• Database Link:
  P30968
• Background: Gonadotropin releasing hormone (GnRH) receptor is a protein of 54-60 kDa. GnRH receptor contains seven hydrophobic transmembrane domains connected by hydrophilic extracellular and intracellular loops characteristic of G-protein coupled receptors. GnRH stimulates the gonadotrophs of the anterior pituitary to secrete luteinizing hormone (LH) as well as follicle-stimulated hormone (FSH). The receptor contains of seven hydrophobic transmembrane domains connected by hydrophillic extracellular, and intracellular loops characteristic of G-protein coupled receptors.
• Synonyms: Gonadotropin-releasing hormone receptor, GnRH-R, GRHR

Reference Data