Plakophilin 1 (PKP1) Mouse Monoclonal Antibody [Clone ID: PP1-5C2]

SKU BM5110

Plakophilin 1 (PKP1) mouse monoclonal antibody, clone PP1-5C2, Supernatant

Product Data

• Clone Name: PP1-5C2
• Application: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA. ; Western blot. ; Immunocytochemistry.; Immunohistochemistry on Cryostat Sections: Ready-to-use. ; Incubation time: 1 h at RT. ; Immunohistochemistry on Paraffin Embedded Sections: Ready-to-use. ; Use pretreatment with 0.001% trypsin together with microwave method. ; Incubation time: overnight at 2-8°C. ; Recommendations: ; For better resolution on frozen sections preincubation (directly after fixation) with 0.05-0.2% Triton X-100, for 5-10 min, depending on tissue type, is recommended (See Ref.3). ; For delicate tissues like liver and gastrointestinal material preincubation with saponin is preferred to Triton. For Immunolocalization studies on electron microscopic level preincubation with 0.1-1% saponin is recommended.
• Reactivity: Bovine, Human, Porcine, Xenopus
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Plakophilin 1 ("band 6 polypeptide" of bovine muzzle epidermis)
• Specificity: Antibody recognizes Plakophilin 1 in diverse stratified epithelia, in complex epithelia (e.g. urothelium of the bladder and tracheal epithelium) and in glandular duct epithelia; squamous cell carcinoma are positive, whereas all adenocarcinoma tested so far are negative.; Polypeptide Reacting: Plakophilin 1: calculated mol. wt. 80,496 (human) and 80,180 (bovine). ; Tested Reactivities on Human Cultured Cell Lines: HaCaT, A-431.
• Buffer: State: Supernatant; State: Liquid Cell Culture Supernatant; Preservative: 0.09% Sodium Azide
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: plakophilin 1
• Database Link:
  Q13835
• Background: Plays a role in formation of desmosomal plaques and is found in desmosomes of most simple and stratified epithelia. Not found in cell types that have non-epithelial desmosomes. Absent in fibroblasts and other connective tissue types, including sarcomas.
• Synonyms: Plakophilin-1, Band 6 protein, B6P

Reference Data