MHC Class I H-2 (b,d,q,h4,m,w16) Rat Monoclonal Antibody [Clone ID: ER-HR52]

SKU BM4093

MHC Class I H-2 (b,d,q,h4,m,w16) rat monoclonal antibody, clone ER-HR52, Purified

Product Data

• Clone Name: ER-HR52
• Application: FC, IHC
• Recommended Dilution: Immunohistochemistry on Frozen Sections: 1 µg/ml (1/200).; Recommended Positive Control: Mouse Spleen.; Does not react on routinely processed Paraffin Sections.; Has been described to work in FACS.
• Reactivity: Mouse
• Antibody Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Mouse macrophage precursor cells
• Specificity: ER-HR52 is useful for detecting MHC class I antigens. It is therefore a valuable tool for studying cytotoxic T-cell interactions with class I positive antigen presenting cells.; The antigen is expressed by all somatic cells at varying levels. ER-HR52 detects MHC class I antigens of various haplotypes (see below).; The antigen is found on all somatic cells in all organs sections though at varying levels. Lymphocytes are highly positive, whereas fibroblasts and neurons show only a low level of antigen expression.
• Buffer: PBS, pH 7.2 containing 5 mg/ml BSA as a stabilizer and 0.09% Sodium Azide as a preservative; State: Purified; State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore by adding 0.5 ml distilled water.
• Concentration: 0.2 mg/ml
• Purification: Affinity Chromatography.
• Conjugation: Unconjugated
• Storage: Store the original vial at 2-8°C and the reconstituted stock solution (in aliquots) at -20°C.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Ambient
• Note: Protocol: Protocol with Frozen, ice-cold Acetone-Fixed Sections:; The whole procedure is performed at room temperature; 1. Wash in PBS; 2. Block endogenous peroxidase; 3. Wash in PBS; 4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber; 5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber; 6. Wash in PBS; 7. Incubate with secondary antibody (peroxidase-conjugated goat anti rat IgG (H+L) minimal-cross reaction to mouse) for 1h in a humid chamber; 8. Wash in PBS; 9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min.; 10. Wash in PBS; 11. Counterstain with Mayer’s hemalum.

Reference Data