pan Cytokeratin Mouse Monoclonal Antibody [Clone ID: Lu5]

SKU BM4068

pan Cytokeratin mouse monoclonal antibody, clone Lu5, Aff - Purified

Product Data

• Clone Name: Lu5
• Application: IHC
• Recommended Dilution: Immunohistochemistry on Frozen Sections: 0.4-0.8 μg/ml (1/500-1/1000).; Immunohistochemistry on Paraffin Sections: 2 μg/ml (1/200). Proteinase K pretreatment for antigen retrieval is recommended.; Suggested Positive Control: Human tonsil.
• Reactivity: Amphibian, Bovine, Chicken, Human, Rat
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human lung cancer cell line
• Specificity: Monoclonal antibody BM4068 recognizes most subtypes of cytokeratins in cryofixed and paraffin sections and is ideally suited as a first order pan-epithelial marker: Clone Lu-5 stains an intracytoplasmic, formaldehyde (paraffin embedding) resistant epitope on the surface of cytokeratin filaments.; The epitope recognized by this antibody is a formalin-resistant marker of great value in tumour diagnosis, located on the surface of cytokeratin filaments. It has been preserved during vertebrate evolution and can be shown in all species from amphibia to man. The epitope is present in most cytokeratin polypeptides of both the acidic (type I) and basic (type II) subfamily but does not occur in other cytoskeletal proteins.; The epithelial specificity and the broad tissue and species cross-reactivity provide an excellent probe for the differential diagnosis of epithelial versus mesenchymal tumours, large cell lymphomas and neural tumours.
• Buffer: PBS, pH 7.2; State: Aff - Purified; State: Lyophilized purified Ig fraction; Stabilizer: 5 mg/ml BSA; Preservative: 0.05% Kathon
• Reconstitution Method: Restore by adding 0.5 ml distilled water. This stock solution contains 0.4 mg/ml IgG.
• Concentration: 0.4 mg/ml (after reconstitution)
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.; After reconstitution store the antibody undiluted at 2-8°C for one month ; or (in aliquots) at -20°C long term.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Ambient
• Synonyms: pan Keratin, Cytokeratin pan-reactive
• Note: Lu-5 has been tested on a wide variety of healthy and tumorous human tissues. 95% of epithelial tumours regardless of their localisation and grade of differentiation were detected (Von Overbeck et al. 1985). It is currently used for differentiating epithelial and mesothelial tumours from mesenchymal tumours, large cell lymphomas and neuronal tumours.

Reference Data