Fibroblasts (Pan Reticular) Rat Monoclonal Antibody [Clone ID: ER-TR7]
Fibroblasts (Pan Reticular) rat monoclonal antibody, clone ER-TR7, Purified
|Recommended Dilution||Immunohistochemistry on Frozen Sections: 1 µg/ml (1/400) freshly prepared for Mouse tissue and 4 µg/ml (1/100) on Human/Porcine tissue.
Recommended Positive Control: Mouse spleen.
Does not react on routinely processed Paraffin Sections.
Has been described to work in FACS.
|Reactivities||Human, Mouse, Porcine|
|Immunogen||Murine thymic reticulum.
Epitope: The antigen has not been fully characterized.
The epitope may be part of reticulin.
|Specificity||Reacts with Mouse reticular fibroblasts, reticular fibres and Human tonsil.
ER-TR7 detects an antigen present in and produced by reticular fibroblasts. The recognized antigen is most likely distinct from Laminin, Fibronectin, Collagen types I-IV, Heparan Sulfate Proteoglycan, Entactin, and Nidogen.
The antibody is useful to stain the microanatomy of various organs, in particular the connective tissue framework in lymphoid organs. The antibody also stains subendothelial deposits in the plaque area of atherosclerotic plaques.
Isolated Cells: The antigen is found in the cytoplasm of fibroblast cell lines.
Tissue Sections: The antigen is expressed in connective tissues which form a supporting network between parenchymal cells of all organs tested so far (see table below). Thus, the supportive mesenchymal structures of larger vessels can be studied. In spleen, a very clear delineation of red and white pulp is obtained. Capsule, sinuses, follicules, paracortex and medullary cords are also clearly delineated in lymph nodes.
|Formulation||Stock solution contains PBS, pH 7.2 with 5 mg/ml BSA as a stabilizer and 0.09% Sodium Azide as preservative
State: Lyophilized purified IgG fraction
|Reconstitution Method||Restore with 0.5 ml distilled water.|
|Concentration||0.4 mg/ml (after reconstitution)|
|Storage||Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Background||Connective tissue consists of a ground of glycosaminoglycans, proteoglycans and glycoproteins through which various fibres run. These fibres can be collagenous, elastic or reticular. Reticular fibres are composed from the family of collagen proteins and give tensile strength. These fibres are made by reticular fibroblasts.
Fibroblasts are the least specialized cells in the connective-tissue family. They are dispersed in connective tissue throughout the body, where they secrete a nonrigid extracellular matrix (ECM) that is rich in type I and/or type III collagen. Conective tissue consists of glycosaminoglycans, proteoglycans and glycoproteins through which various fibres run. These fibres can be collagenous, elastic or reticular. Reticular fibres are composed from the family of collagen proteins and give tensile strength. These fibres are made by reticular fibroblasts. The activation of fibroblasts by inflammatory stimuli results in their migration, proliferation and deposition of extracellular matrix components, important features involved in both wound healing and fibrosis.
|Synonyms||Fibroblast Marker, Fibroblasten|
|Note||Protocol: Protocol with frozen, Ice-cold Acetone-Fixed Sections:
(The whole procedure is performed at room temperature).
1. Wash in PBS.
2. Block endogenous peroxidase.
3. Wash in PBS.
4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber.
5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber.
6. Wash in PBS.
7. Incubate with secondary antibody (peroxidase-conjugated goat anti rat IgG (H+L) minimal-cross reaction to mouse) for 1h in a humid chamber.
8. Wash in PBS.
9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min.
10. Wash in PBS.
11. Counterstain with Mayers hemalum.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.