Adgre1 Rat Monoclonal Antibody [Clone ID: BM8]

CAT#: BM4007F

Adgre1 rat monoclonal antibody, clone BM8, FITC

Product Images

Mouse anti-Macrophage F4/80 antigen antibody. (5 ug/ml) on Raw 204.4 cells. Cells were fixed in 1% PFA, permeabilized in 0.25%Triton X 100 in PBS, blocked in 1% BSA in PBS.

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  Mouse anti-Macrophage F4/80 antigen antibody. on thioglycollate elicited mouse peritoneal macrophages. Purple: ; Green: Isotype control SM15F; Percentages reflect % positive after subtraction of negative control, using M1 marker.

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  Comparison of different mature macrophage markers

Specifications

Product Data

• Clone Name: BM8
• Applications: FC, IF
• Recommended Dilution: Has been described to work in FACS.
  Suggested Positive Control: Mouse monocytes.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Cultured macrophages
• Specificity: This antibody recognizes the F4/80 antigen on major subpopulations of resident tissue macrophages (Schaller et al. 2002).
  Antigen Distribution
  Isolated Cells: The antigen is expressed in vitro on over 80% of M-CSF stimulated bone marrow derived macrophages, after a few days of culture. It is absent from granulocytes, lymphocytes and thrombocytes.
  Tissue Sections: The antigen is detected on tissue fixed macrophages in all organs tested so far (spleen, lymph nodes, thymus, liver, skin) except lung. It is also present on Langerhans cells in the skin and Kupffer cells in the liver. In complete Freund's adjuvant induced granulomas, the antigen is expressed by inflammatory macrophages, but is absent from epitheloid cells.
• Formulation: PBS, pH 7.2
  Label: FITC
  State: Liquid purified Ig fraction
  Stabilizer: 10 mg/ml BSA
  Preservative: 0.09% Sodium Azide
• Concentration: 0.1 mg/ml
• Purification: Affinity Chromatography
• Conjugation: FITC
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Gene Name: adhesion G protein-coupled receptor E1
• Database Link:
  Entrez Gene 13733 Mouse
  UniProt ID Q61549
• Background: The antigen expression increases upon maturation of macrophage precursors in bone marrow and blood as well as in ontogeny. BM8 is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas and has been shown to be a unique histological marker of the progression from peri-insulitis to β-cell destruction and diabetes in a mouse diabetes model.
• Synonyms: Emr1, Gpf480