Corticotropin Releasing Factor (CRH) Mouse Monoclonal Antibody [Clone ID: 4H9]

SKU BM357

Corticotropin Releasing Factor (CRH) mouse monoclonal antibody, clone 4H9, Purified

Product Data

• Clone Name: 4H9
• Application: ELISA, IHC
• Recommended Dilution: Immunohistochemistry on Frozen sections.; Immunohistochemistry on Paraffin sections: 1/100 - 1/7000; Microwave pretreatment is required. Enzyme digestion with 0.1% trypsin may also be necessary.; ELISA: This antibody is suitable for use in a sandwich ELISA with clone 5D5 (BM356).
• Reactivity: Human
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Corticotrophin Releasing Factor conjugated to Bovine Serum Albumin.
• Specificity: This antibody is specific for corticotrophin releasing factor (CRF), a hormone found mainly in the hypothalamus that regulates the release of corticotrophin from the pituitary gland. The unique lysine residue (Lys-36) is likely to be involved in the antigenic determinant as lysine modification by CA blocks binding completely. Human serum, containing CRF-binding protein, also inhibits the binding of this antibody to CRF.
• Buffer: PBS containing 0.09% Sodium Azide; State: Purified; State: Liquid purified IgG
• Concentration: lot specific
• Purification: Affinity chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: corticotropin releasing hormone
• Database Link:
  P06850
• Background: CRF precursor is a 41-amino acid peptide derived from a 196-amino acid preprohormone. CRF is a neuroendocrine hormone secreted by the paraventricular nucleus (PVN) of the hypothalamus and is a key player in the hypothalamic pituitary adrenal axis. It is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. Neuronal CRF is important in a variety of diverse roles including, memory, depression, stress, kidney function, parturition, metabolism and feeding (to mention a few).
• Synonyms: CRF, CRH, Corticotropin-Releasing Hormone, Corticotropin-Releasing Factor

Reference Data