ICAM1 Mouse Monoclonal Antibody [Clone ID: MEM-111]

CAT#: BM2448P

ICAM1 mouse monoclonal antibody, clone MEM-111, Purified

Product Images

Western blottin analysis of CD54 expression in TNF-alpha activated (A) and nonactivated (B) HUVEC cells by antibody MEM-111. Lower bands represent tubulin as a loading control.

Specifications

Product Data

• Clone Name: MEM-111
• Applications: ELISA, FC, IHC, WB
• Recommended Dilution: Flow cytometry: Recommended dilution: 0.5-4 μg/ml.
  Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: thymus, RE cells.
  Western blotting: Positive control: RAJI cell line K562 leukemia cell line, JY cell line, aa ctivated T-lymphocytes, non-reducing conditions.
• Reactivities: Human, Rat, Bovine
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Raji Human Burkitt's lymphoma cell line.
• Specificity: Clone MEM-111 reacts with CD54.
• Formulation: PBS, pH~7.4
  State: Purified
  State: Liquid purified Ig fraction (95% pure by SDS-PAGE)
  Preservative: 15mM Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Gene Name: intercellular adhesion molecule 1
• Database Link:
  Entrez Gene 3383 Human
  UniProt ID P05362
• Background: ICAM-1 (MW 90 kD) is a member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells.
  CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.
• Synonyms: ICAM-1