Endomucin (EMCN) Rabbit Polyclonal Antibody

CAT#: AP60011PU-L

Endomucin (EMCN) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis using anti-Human Endomucin antibody. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions. Lane 1: MWM (kDa); Lane 2: rh sEndomucin.

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  Immunofluorescence staining (green) of cryo-sections of Human foreskin (fixed 15 min in 4% PFA) with anti-Human Endomucin antibody (5 ug/ml) and counter staining of nuclei with DAPI (blue) and double staining wit anti-CD31 (right panel). Performed by the research group of Prof. Dr. J. Wilting and Dr. K. Buttler, University Göttingen, Germany.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1-5 µg/ml.
  Immunofluorescence/Immunohistochemistry: 1-5 µg/ml (Cryo).
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Highly pure (>95%) recombinant Human soluble Endomucin (Asn18 - Ser190) derived from E. coli (Cat.-No AR31181PU-N).
• Specificity: This antibody detects Human Endomucin (Asn18 - Ser190).
  Other species not tested.
• Formulation: PBS
  State: Purified
  State: Lyophilized purified Ig fraction
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml. Centrifuge vial prior to opening.
• Purification: Protein A Chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 month or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: endomucin
• Database Link:
  Entrez Gene 51705 Human
  UniProt ID Q9ULC0
• Background: Endomucin (endothelial sialomucin; also Endomucin-1/2 and Mucin-14) is an 80 - 120 kDa glycoprotein member of the Endomucin family of proteins. It is expressed on endothelial cells and depending upon its glycosylation pattern, can serve as either a pro- or anti-adhesive molecule. Mouse Endomucin precursor is 261 amino acids in length. It is type I transmembrane protein that contains a 170 aa extracellular domain (ECD) (aa 21 - 190) and a 50 aa cytoplasmic region. Three splice variants exist in the ECD. One shows a deletion of aa 91 - 141, a second shows a one aa substitution for aa 91 - 129, and a third shows a one aa substitution for aa 129 - 142. Over aa 21 - 90, mouse Endomucin shares 60% and 30% aa identity with rat and human Endomucin, respectively.
• Synonyms: EMCN, EMCN2, MUC14, Endomucin-2, Mucin-14, Gastric cancer antigen Ga34