C6orf150 (MB21D1) (Center) Rabbit Polyclonal Antibody

CAT#: AP50874PU-N

C6orf150 (MB21D1) (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis in MDA-MB231 cell line lysates using CF150 Antibody (Center) (35ug/lane). This demonstrates the CF150 antibody detected the CF150 protein (arrow).

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  Flow Cytometric analysis of MDA-MB231 cells using CF150 Antibody (Center) (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/1000.
  Flow Cytometry: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 266-295 amino acids from the Central region of Human CF150
• Specificity: This antibody recognizes Human C6orf150 (CF150). Other species not tested.
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% (W/V) Sodium Azide
• Concentration: lot specific
• Purification: Protein A Chromatography followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 58814 Da
• Gene Name: Mab-21 domain containing 1
• Database Link:
  Entrez Gene 115004 Human
  UniProt ID Q8N884
• Background: Function: Nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP. Catalysis involves both the formation of a 2',5' phosphodiester linkage at the GpA step and the formation of a 3',5' phosphodiester linkage at the ApG step, producing c[G(2',5')pA(3',5')p]. Has antiviral activity by acting as a key cytosolic DNA sensor, the presence of double- stranded DNA (dsDNA) in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production.
  Cellular Location: Cytoplasm, cytosol.
  Tissue Location: Expressed in the monocytic cell line THP1.
• Synonyms: C6orf150; cGAS; h-cGAS