RPL22 (C-term) Goat Polyclonal Antibody

SKU AP33461PU-N

RPL22 (C-term) goat polyclonal antibody, Aff - Purified

Product Data

• Application: ELISA, IHC
• Recommended Dilution: ELISA: 1/16000 (Detection Limit). ; Western blot: Preliminary experiments gave bands at approx 17-18kDa and 25kDa in Human Liver lysates after 0.5µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 14.8kDa according to NP_000974.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). This batch is not expected to cross-react with Mouse and Rat in Western blot.; Immunohistochemistry on Paraffin Sections: ; 3-6 µg/ml. In paraffin embedded Human Brain (Cortex) shows cytoplasm staining in neuronal bodies.; 3.75 µg/ml. In paraffin embedded Human Thyroid, Uterus and Testis.
• Reactivity: Human, Mouse, Rat
• Antibody Host: Goat
• Clonality: Polyclonal
• Immunogen: Synthetic peptide (C-SKESYELRYFQINQ) from C-terminus of Human RPL22
• Specificity: This antibody recognizes the C-term of RPL22.
• Buffer: Tris saline buffer, pH~7.3; State: Aff - Purified; State: Liquid purfied IgG fraction; Stabilizer: 0.5% BSA; Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: ribosomal protein L22
• Database Link:
  P35268
• Synonyms: EBER-associated protein, EAP, HBp15

Reference Data