Amyloid beta A4 protein / APP (556-575) Chicken Polyclonal Antibody
Product Data | |
Application | IF, IHC |
---|---|
Recommended Dilution | Immunocytochemistry. Immunohistochemistry. Recommended Dilutions: 1/500-1/1000 for Immunohistochemistry and Immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells. Quality Control: This anti-Peptide antibody mixture was analyzed by Immunohistochemistry (1/1000 dilution) using Fluorescein-labeled Goat anti-Chicken IgY (1/500 dilution, Cat.-No AP31795FC-N) as the secondary reagent. |
Reactivity | Human, Mouse, Rat |
Antibody Host | Chicken |
Isotype | IgY |
Clonality | Polyclonal |
Immunogen | Chickens were immunized with synthetic peptide APP#5 conjugated to keyhole limpet hemocyanin (KLH). The sequence of APP peptide #5 is EEI QDE VDE LLQ KEQ NYS DD, which corresponds to residues #556-575 of the Human gene product (NP_000475.1)]. Identical sequences are also found in the Mouse and Rat homologs of APP. |
Specificity | Recognizes Amyloid Precursor Protein (APP), Peptide #5. |
Buffer | Sodium Phosphate-Buffered (10 mM) isotonic (0.9%, w/v) Saline (PBS, pH 7.2) State: Aff - Purified State: Liquid purified (filter sterilized) IgY fraction Preservative: 0.02% (w/v) Sodium Azide |
Concentration | lot specific |
Purification | Affinity Chromatography using a peptide column. Preparation: After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 100 ug/ml. Finally, equal volumes of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized. |
Conjugation | Unconjugated |
Storage | Store the antibody undiluted in the dark at 2-8°C. |
Stability | Shelf life: one year from despatch. |
Shipping | Blue Ice |
Gene Name | amyloid beta precursor protein |
Database Link | |
Synonyms | Alzheimer disease amyloid protein, Amyloid Precursor Protein, ABPP, APPI, PreA4, Cerebral vascular amyloid peptide, CVAP |
Reference Data |
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