MAPKAP Kinase 2 (MAPKAPK2) (44-55) Goat Polyclonal Antibody

SKU AP32811PU-N

MAPKAP Kinase 2 (MAPKAPK2) (44-55) goat polyclonal antibody, Aff - Purified

Product Data

• Application: ELISA, IP, WB
• Recommended Dilution: Peptide ELISA: 1/32000 (Detection Limit).; Western blot: 0.5-2 μg/ml. In transfected HEK293 transiently expressing either Mouse MK2 fused to GFP or the similar Mouse MK5 fused to GFP, only the expected band was observed in the MK2 lane after AP32810PU-N and AP32811PU-N labeling. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.; Immunoprecipitation: MK2/MK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced wiith MK2 or empty vector were lysed from confluent plates and used for IP with 1.5 µg AP32810PU-N or AP32811PU-N. ; Western blots of the Immunoprecipitation were labelled with Rabbit anti-MK2 (CST). An approx 45kDa of MK2 is only precipitated from lysates of those KO MEFs that have been rescued by the MK2 expression construct as described in PMID: 19743408. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.
• Reactivity: Human, Mouse, Rat
• Antibody Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence (44-55) from the internal region of the protein sequence according to NP_004750.1; NP_116584.2.
• Specificity: This antibody is expected to recognize both reported isoforms (NP_004750.1; NP_116584.2).
• Buffer: Tris saline, pH~7.3; State: Aff - Purified; State: Liquid purified Ig fraction; Stabilizer: 0.5% BSA; Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: mitogen-activated protein kinase-activated protein kinase 2
• Database Link:
  P49137
• Synonyms: MAPK-activated protein kinase 2, MAPKAP kinase 2, MAPKAPK-2, MK2, MAPKAPK2

Reference Data