ATG4A (369-398) Rabbit Polyclonal Antibody

SKU AP32201PU-N

ATG4A (369-398) rabbit polyclonal antibody, Purified

Product Data

• Application: IHC, WB
• Recommended Dilution: ELISA: 1/1,000. ; Western blot: 1/100-1/500. ; Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivity: Human, Mouse
• Antibody Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 369~398 amino acids from the C-term of Human APG4A
• Specificity: This antibody recognizes Human and Mouse ATG4A (369-398).; Other species not tested.
• Buffer: PBS containing 0.09% (W/V) Sodium Azide as preservative; State: Purified; State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Saturated Ammonium Sulfate precipitation followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Shipping: Blue Ice
• Predicted Protein Size: 45378 Da
• Gene Name: autophagy related 4A cysteine peptidase
• Database Link:
  Q8WYN0
• Background: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG4A is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP.
• Synonyms: Cysteine protease ATG4A, ATG4A, APG4A, AUTL2

Reference Data