BOB1 (POU2AF1) (C-term) Goat Polyclonal Antibody

SKU AP32080PU-N

BOB1 (POU2AF1) (C-term) goat polyclonal antibody, Aff - Purified

Product Data

• Application: ELISA, IHC, WB
• Recommended Dilution: Peptide ELISA: 1/8000 (Detection Limit).; Western Blot: 1-2 µg/ml. In transfected HEK293 transiently expressing full-length Human POU2AF1 (myc and DYKDDDDK tagged), a band of approx. 38kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody.; Immunohistochemistry on Paraffin Sections: 3-5 μg/ml. In paraffin embedded Human Spleen shows nuclear staining in the germinal center with stronger nucleoli staining in some of the B cells. ; Purchase the Positive Control! The transfected lysate used to QC this product is available for sale.
• Reactivity: Bovine, Canine, Human
• Antibody Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the C-Terminus of Human POU2AF1 (NP_006226.2)
• Specificity: This antibody detects POU2AF1 / BOB1 at C-term.
• Buffer: Tris saline, pH~7.3; State: Aff - Purified; State: Liquid purified IgG fraction; Stabilizer: 0.5% BSA; Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Shipping: Blue Ice
• Gene Name: POU class 2 associating factor 1
• Database Link:
  Q16633
• Synonyms: OBF1, OBF-1, OCA-B, OCAB

Reference Data