Penk Goat Polyclonal Antibody

CAT#: AP32079PU-N

Penk goat polyclonal antibody, Aff - Purified

Product Images

PENK Antibody staining of PFA-perfused cryosection of Rat Striatum at 0.02 ug/ml. HRP-staining with Ni-DAB after Biotin-SP-anti-goat amplification. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med., Budapest, Hungary.

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  PENK Antibody staining of PFA-perfused cryosection of Mouse Stria terminalis at 0.02 ug/ml. HRP-staining with Ni-DAB after Biotin-SP-anti-goat amplification. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med., Budapest, Hungary.

Specifications

Product Data

• Applications: ELISA, IHC
• Recommended Dilution: Peptide ELISA: 1/32000 (Detection Limit).
  Western Blot: Preliminary experiments gave an approx 40kDa band in Rat Brain lysates after 0.2 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 30.8kDa according to Mouse NP_001002927.1 and 30.9kDa according to Rat NP_058835.1.
  The 40kDa band was successfully blocked by incubation with the immunizing peptide.
  Immunohistochemistry on Frozen Sections: 0.02-0.05 μg/ml.
  Frozen section of Rat Brain (Striatum) shows staining of dense enkephalinergic axon plexus.
  Frozen section of the Mouse Brain (Stria terminalis) shows staining of a dense enkephalinergic fibre network in the bed nucleus.
• Reactivities: Mouse, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Enkephalin antibody was raised against a synthetic peptide of Enkephalin.
• Specificity: This antibody recognizes Proenkephalin (PENK).
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified IgG fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: preproenkephalin
• Database Link:
  Entrez Gene 18619 Mouse
  UniProt ID P22005
• Synonyms: preproenkephalin; proenkephalin