Penk Goat Polyclonal Antibody
| Product Data | |
| Application | ELISA, IHC |
|---|---|
| Recommended Dilution | Peptide ELISA: 1/32000 (Detection Limit). Western Blot: Preliminary experiments gave an approx 40kDa band in Rat Brain lysates after 0.2 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 30.8kDa according to Mouse NP_001002927.1 and 30.9kDa according to Rat NP_058835.1. The 40kDa band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry on Frozen Sections: 0.02-0.05 μg/ml. Frozen section of Rat Brain (Striatum) shows staining of dense enkephalinergic axon plexus. Frozen section of the Mouse Brain (Stria terminalis) shows staining of a dense enkephalinergic fibre network in the bed nucleus. |
| Reactivity | Mouse, Rat |
| Antibody Host | Goat |
| Clonality | Polyclonal |
| Immunogen | Enkephalin antibody was raised against a synthetic peptide of Enkephalin. |
| Specificity | This antibody recognizes Proenkephalin (PENK). |
| Buffer | Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified IgG fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide |
| Concentration | lot specific |
| Purification | Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide |
| Conjugation | Unconjugated |
| Storage | Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Gene Name | preproenkephalin |
| Database Link | |
| Synonyms | preproenkephalin; proenkephalin |
| Reference Data | |
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