Smgc Goat Polyclonal Antibody

SKU
AP31966PU-N
Smgc goat polyclonal antibody, Aff - Purified
$565.00
5 Days*
Specifications
Product Data
Application ELISA, IF
Recommended Dilution
Peptide ELISA: 1/16000 (Detection limit).
Western blot: Preliminary experiments gave bands at approx 65kDa and 70kDa in Mouse fetal Lung lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 74.4kDa according to NP_945121.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Immunocytochemistry: 4 µg/ml. Positive staining of select epithelial cells in the murine Submanibular gland at E18. Data provided by Melinda Larsen, State University of New York, Albany, NY.
Reactivity Mouse
Antibody Host Goat
Clonality Polyclonal
Immunogen Synthetic peptide: KLEPKYENPTNGS with a Cysteine residue linker, corresponding to internal sequence amino acids 518-530 of Mouse Muc19 (NP_945121.1).
Specificity This antibody recognizes Mouse Muc19 /Smgc.
Other species not tested.
Buffer Tris saline, pH~7.3
State: Aff - Purified
State: Liquid purified Ig fraction
Stabilizer: 0.5% BSA
Preservative: 0.02% Sodium Azide
Concentration lot specific
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Conjugation Unconjugated
Storage

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.

Stability Shelf life: one year from despatch.

Shipping Blue Ice
Gene Name submandibular gland protein C
Database Link
Background Detected in terminal tubule cells of the submandibular gland (at protein level). Expressed in submandibular salivary glands of 3-day-old males but not adults. Expression in adult submandibular glands is restricted to females. Isoform 5 is expressed in both 3-day-old and adult sublingual glands.
Synonyms Submandibular gland protein C, Muc19
Reference Data
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