Tyrosine Hydroxylase (TH) Chicken Polyclonal Antibody

SKU AP31824PU-N

Tyrosine Hydroxylase (TH) chicken polyclonal antibody, Aff - Purified

Product Data

• Application: IF, IHC, WB
• Recommended Dilution: Western Blot.; Immunocytochemistry.; Immunohistochemistry.; Recommended Dilutions:; 1/2000-1/5000 for Western Blots.; 1/1000-1/2000 for Immunohistochemistry and Immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells.; Quality Control: Both of the the antibodies was analyzed by Immunohistochemistry (at 3 µg/ml) using Fluorescein-labeled Goat anti-Chicken IgY (1/500 dilution, Cat.-No AP31795FC-N) as the secondary reagent.
• Reactivity: Human, Mouse
• Antibody Host: Chicken
• Isotype: IgY
• Clonality: Polyclonal
• Immunogen: Two antipeptide antibodies were generated in Chickens against sequences shared between the Mouse (P24529) and Human (P07101) gene products.; Production: After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.2 mg/ml. Finally, equal volumes of both of these affinity purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
• Specificity: Recognizes Tyrosine Hydroxylase (TYH).
• Buffer: 10mM PBS, pH 7.2 containing 0.02% Sodium Azide as preservative.; State: Aff - Purified; State: Liquid purified (filter sterilized) IgY fraction.
• Concentration: lot specific
• Purification: Affinity Chromatography using a peptide column.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted in the dark at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: tyrosine hydroxylase
• Database Link:
  P07101
• Background: Human TYH (EC 1.14.16.2) is a 58,523 dalton protein (528 amino acids) responsible for the enzymatic conversion of L-tyrosine to L-DOPA (dihydroxyphenylalanine). This enzyme is expressed in all catecholaminergic neurons of the CNS and PNS. In the CNS, TYH-positive neurons can be found within the substantia nigra, ventral tegmental area, locus ceruleus, and hypothalamus. In the PNS, TYH-positive neurons can be found within the sympathetic chain, pre-vertebral ganglia and the adrenal medulla.
• Synonyms: Tyrosine 3-hydroxylase, TYH

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Parkinson's disease, Tyrosine metabolism