GFAP Chicken Polyclonal Antibody

CAT#: AP31806PU-N

GFAP chicken polyclonal antibody, Aff - Purified

Product Images

Dissociated culture of Rat cerebral cortical neurons and astrocytes (red staining). The nuclei of some neuronal cells (blue staining) are visible. Picture courtesy of Dr. Gerry Shaw, University of Florida.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot.
  Immunocytochemistry.
  Immunohistochemistry.
  Recommended Dilutions:
  1/10000-1/20000 for Western blots.
  1/2000-1/5000 for Immunohistochemistry and Immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells.
  Quality Control: This antibody was analyzed by Immunohistochemistry (at dilution of 1/5000) using Fluorescein-labeled Goat anti-Chicken IgY (1/500 dilution, Cat.-No AP31795FC-N) as the secondary reagent.
• Reactivities: Human, Mouse, Rat
• Host: Chicken
• Isotype: IgY
• Clonality: Polyclonal
• Immunogen: Recombinant Human GFAP (expressed in bacteria).
  After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks.
• Specificity: Recognizes Glial Fibrillary Acidic Protein (GFAP).
• Formulation: 10 mM PBS, pH 7.2 containing 0.02% Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid purified (filter sterilized) IgY fraction.
• Concentration: 2 mg/ml (based on absorbance at 280 nm)
• Purification: Affinity Chromatography using a peptide column.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  
• Stability: Shelf life: one year from despatch.
• Gene Name: glial fibrillary acidic protein
• Database Link:
  Entrez Gene 14580 MouseEntrez Gene 24387 RatEntrez Gene 2670 Human
  UniProt ID P14136
• Background: Human GFAP is a 49,749 dalton protein (432 amino acids) expressed by astrocytes of the central nervous system. GFAP is an intermediate filament protein and acts as an intra-cellular structural component of the astrocytic cytoskeleton. During embryonic and fetal life, GFAP is also expressed by radial glial cells of the CNS. Rare mutations of the GFAP gene in humans result in Alexander’s disease, one of the leukodystrophies.
• Synonyms: Glial Fibrillary Acidic Protein

Reference Data

• Protein Families: ES Cell Differentiation/IPS