GAD67 (GAD1) Chicken Polyclonal Antibody

SKU AP31805PU-N

GAD67 (GAD1) chicken polyclonal antibody, Aff - Purified

Product Data

• Application: IF, IHC, WB
• Recommended Dilution: Western Blot.; Immunocytochemistry.; Immunohistochemistry.; Recommended Dilutions:; 1/2000-1/5000 for Western blots.; 1/1000-1/2000 for Immunohistochemistry and Immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells.; Quality Control: This antibody was analyzed by Immunohistochemistry (at a concentration of 1 µg/ml) using Fluorescein-labeled Goat anti-Chicken IgY (1/500 dilution, Cat.-No AP31795FC-N) as the secondary reagent.
• Reactivity: Human, Mouse, Rat
• Antibody Host: Chicken
• Isotype: IgY
• Clonality: Polyclonal
• Immunogen: Synthetic peptide KLH conjugated corresponding to a region near the C-terminus of this gene product, and was 100% conserved between the Human (Q99259), Mouse (P48318) and Rat (NP_058703) gene products.; After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity purified using a peptide column.
• Specificity: Recognizes Glutamic Acid Decarboxylase (GAD1/GAD67).
• Buffer: 10mM PBS (0.9%, w/v), pH 7.2 containing 0.02% Sodium Azide as preservative.; State: Aff - Purified; State: Liquid purified (0.45 µm filter sterilized) IgY fraction.
• Concentration: lot specific
• Purification: Affinity Chromatography using a peptide column.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted in the dark at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: glutamate decarboxylase 1
• Database Link:
  Q99259
• Background: Human Glutamic Acid Decarboxylase (GAD-67), [EC 4.1.1.15] is a 66,987 dalton protein (594 amino acids) selectively expressed in a subpopulation of GABAergic neurons of the CNS. It catalyzes the decarboxylation of glutamic acid, forming the inhibitory neurotransmitter β-amino butyric acid (GABA). It is also know as GAD-1.
• Synonyms: Glutamate decarboxylase 1, GAD-67

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism, beta-Alanine metabolism, Butanoate metabolism, Metabolic pathways, Taurine and hypotaurine metabolism, Type I diabetes mellitus