DNMT3B (1-50) Rabbit Polyclonal Antibody

SKU AP31351PU-N

DNMT3B (1-50) rabbit polyclonal antibody, Purified

Product Data

• Application: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA: 1/20000.; Immunofluorescence: 1/100 - 1/500.; Immunohistochemistry on Paraffin Sections: 1/100.; Western Blot: 1/500 - 1/1000.
• Reactivity: Human
• Antibody Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from Human DNMT3B (aa 1-50)
• Specificity: This antibody detects endogenous levels of total DNMT3B protein.
• Buffer: PBS (without Mg²⁺, Ca²⁺), pH 7.4 containing 150 mM Sodium Chloride, 0.02% Sodium Azide and 50% Glycerol.; State: Purified; State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Predicted Protein Size: 95 kDa
• Gene Name: DNA (cytosine-5-)-methyltransferase 3 beta
• Database Link:
  Q9UBC3
• Background: Methylation at the 5'-position of cytosine is the only known naturally occurring covalent modification of the mammalian genome. DNA methylation requires the enzymatic activity of DNA 5-cytosine methyltransferase (Dnmt) proteins, which catalyze the transfer of a methyl group from S-adenosyl methionine to the 5'-position of cytosines residing in the dinucleotide CpG motif, and this methylation results in transcriptional repression of the target gene. The Dnmt enzymes are encoded by independent genes. Dnmt1 is the most abundant, and it preferentially methylates hemimethylated DNA and coordinates gene expression during development. Additional mammalian Dnmt proteins include Dnmt2 and Dnmt3. Dnmt2 lacks the large N-terminal regulator domain of Dnmt1, is expressed at substantially lower levels in adult tissues, and is likely involved in methylating newly integrated retroviral DNA. Dnmt3a and Dnmt3b are encoded by two distinct genes, but both are abundantly expressed in embryonic stem cells, where they also methylate CpG motifs on DNA.
• Synonyms: DNA MTase HsaIIIB, M.HsaIIIB

Reference Data

• Protein Families: Druggable Genome, Embryonic stem cells, Induced pluripotent stem cells, Stem cell - Pluripotency
• Protein Pathways: Cysteine and methionine metabolism, Metabolic pathways