Orexin Receptor 1 (HCRTR1) Goat Polyclonal Antibody

SKU AP31067PU-N

Orexin Receptor 1 (HCRTR1) goat polyclonal antibody, Aff - Purified

Product Data

• Application: ELISA, WB
• Recommended Dilution: Peptide ELISA: Detection Limit: 1/8000.; Western Blot: 0.1-0.3 µg/ml. Approx 48+35kDa band observed in Human Brain lysates. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Silveyra et al, Am J Physiol Endocrinol Metab. 2007 Oct;293(4):E977-85; PMID: 17638707).
• Reactivity: Canine, Human, Mouse, Rat, Sheep
• Antibody Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_001516.1.
• Specificity: This antibody detects Orexin receptor 1.
• Buffer: Tris saline, pH 7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer.; State: Aff - Purified; State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: hypocretin receptor 1
• Database Link:
  O43613
• Background: Orexins are endogeneous peptides that activate Orexin Receptor type 1 and type 2, two closely related G-protein coupled receptors. Orexins-A and -B are hypothalamic peptides derived from a precursor called preproorexin and affect the stimulation of food intake. They act on G protein receptors named orexin receptor-1 (OX1R) and orexin receptor-2 (OX2R), respectively.timulation of these receptors by orexins causes an increase in intracellular calcium levels in hypothalamic cells in vitro. In vivo, orexins control feeding and may also be involved in modulating sleep-wake cycles and other hypothalamic functions.
• Synonyms: Hypocretin receptor type 1, HCRTR1, Ox1r
• Note: Predicted Molecular Weight: 47.5kDa (NP_001516.1)

Reference Data